Self-contained area, arranged in the corner of the laboratory, restricted the entry and exit of personnel with sealed doors, set the room with clean requirements in the place with less interference from personnel, and set the auxiliary room outside. Considering the operation process of microbial test, the detection room is adjacent to the washing and disinfection room and the culture room, which is convenient for the separation of people flow and logistics. In order to control the entry and exit of personnel, only one sealed door is provided to enter the main clean area of microbiology laboratory, and the operators enter the corridor and then the preparation room, and enter the operation area through the first watch and the second watch respectively. Logistics is realized from the transmission window. The air outlet is equipped with high-efficiency filter, and the air outlet is equipped with high-efficiency filter static pressure box. The indoor air supply and exhaust adopts the mode of upper air supply and lower air exhaust, and the indoor air exhaust is arranged on one side without obstacles. The residual pressure valve automatically adjusts the indoor pressure to keep positive pressure clean.